Taxonomic identification report

Sample ID: barcode88_contig1_PE250203-A_REP1_KAPA

Department of Agriculture, Fisheries and Forestry logo

[analysis description + link to github repo]

Facility Hogwarts
Analyst John Doe
Analysis started 2025-05-03 01:27:58
Analysis completed 2025-05-03 01:27:58
Wall time 0:0:0 hours

Result overview

Luthrodes pandava

Outcome: Positive species identification.

Reasoning: [Flag 1A] 1 candidate species matched with high stringency (identity ≥ 98.5%).

Preliminary morphology ID confirmed? True

Flag 7A: Identified species is consistent with preliminary morphology ID Lycaenidae.

Taxa of interest detected? False

Flag 2B: Taxon of interest NOT detected in candidate species

Flag 5.1NA: Assessment of related species is only possible for taxa at rank genus/species

Flag 5.2NA: Assessment of related species is only possible for taxa at rank genus/species

Sample details

Locus COI
Preliminary ID Lycaenidae
Taxa of interest Chilades pandava
Country Australia
Host NA
Sample ID barcode88_contig1_PE250203-A_REP1_KAPA
Query DNA sequence

Analyst evaluation

Identification of candidate species

Flag 1A: Positive species identification - Luthrodes pandava
1 candidate species matched with high stringency (identity ≥ 98.5%)

Candidate hits must meet ONE of these criteria:

Minimum alignment length 400bp
Minimum query coverage 85.0%

Candidate hits have then been classified as follows:

Classification Alignment identity Number of hits Number of species
STRONG MATCH ≥ 98.5% 24 1
MODERATE MATCH ≥ 93.5% NA NA
NO MATCH < 93.5%

Candidate species

Species Hits Identity E-value Database coverage
Luthrodes pandava 24 100.0% 0.0

Analyst evaluation

Taxa of interest (TOI)

This sections shows the taxa of interest (TOI) specified by the sample submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.

Taxon of interest Match rank Match taxon Match species Match accession Match identity Database coverage
Chilades pandava Did not match any candidate

Reference sequence source diversity

This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.

Analyst evaluation

Independent sources

Flag 4A: Reference sequences are from diverse sources and therefore likely to be reliable
Reasoning: Matching sequence records for this species are from >5 independent sources (found 12 sources)

12 Independent Sources

The matching reference sequences for this species have been annotated by 12 independent source(s). A source is considered independent if the author list or publication title is distinct.

Source 1

Hit accession Automated Authors Title Journal
GU372585 False Kim,M.I.
Wan,X.
Kim,M.J.
Jeong,H.C.
Ahn,N.H.
Kim,K.G.
Han,Y.S.
Kim,I.
Phylogenetic relationships of true butterflies (Lepidoptera: Papilionoidea) inferred from COI, 16S rRNA and EF-1alpha sequences Mol. Cells 30 (5), 409-425 (2010)
GU372585 False Kim,M.I.
Kim,I.
Direct Submission Submitted (30-DEC-2009) College of Agricultural & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea

Source 2

Hit accession Automated Authors Title Journal
HQ962268 False iBOL Data Release Unpublished
HQ962268 False Direct Submission Submitted (24-JAN-2011) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd West, Guelph, Ontario N1G2W1, Canada

Source 3

Hit accession Automated Authors Title Journal
MW796551 False Chaianunporn,T.
Chaianunporn,K.
Comparison of butterfly communities (Lepidoptera, Papilionoidea) between agricultural land uses and dipterocarp forest in Khon Kaen Province, Thailand Unpublished
MW796551 False Chaianunporn,T.
Chaianunporn,K.
Direct Submission Submitted (22-MAR-2021) Department of Environmental Science, Khon Kaen University, 123 Mittraphap Road, Mueang Khon Kaen District, Khon Kaen 40002, Thailand

Source 4

Hit accession Automated Authors Title Journal
KF668149 False Fric,Z.
Dickinson,R.
Fetouh,G.
Larsen,T.B.
Schon,W.
Wiemers,M.
First record of the cycad blue, Chilades pandava, in Egypt - a new invasive butterfly species in the Mediterranean region and on the African continent (Lepidoptera: Lycaenidae) Afr Entomol 22 (2), 315-319 (2014)
KF668149 False Fric,Z. Direct Submission Submitted (16-SEP-2013) Ecology & Conservation Biology, Biology Centre CAS, Institute of Entomology, Branisovska 31, Ceske Budejovice, CZ 37005, Czech Republic

Source 5

Hit accession Automated Authors Title Journal
GU076254 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Tracing the origin of Chilades pandava (Lepidoptera, Lycaenidae) found at Kinmen Island using mitochondrial COI and COII genes Unpublished
GU076254 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Direct Submission Submitted (11-OCT-2009) Department of Life Science, National Taiwan Normal University, No. 88, Ting-Chou Rd., Sec. 4, Taipei 116, Taiwan, R.O.C.
GU076257 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Tracing the origin of Chilades pandava (Lepidoptera, Lycaenidae) found at Kinmen Island using mitochondrial COI and COII genes Unpublished
GU076257 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Direct Submission Submitted (11-OCT-2009) Department of Life Science, National Taiwan Normal University, No. 88, Ting-Chou Rd., Sec. 4, Taipei 116, Taiwan, R.O.C.
GU076346 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Tracing the origin of Chilades pandava (Lepidoptera, Lycaenidae) found at Kinmen Island using mitochondrial COI and COII genes Unpublished
GU076346 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Direct Submission Submitted (11-OCT-2009) Department of Life Science, National Taiwan Normal University, No. 88, Ting-Chou Rd., Sec. 4, Taipei 116, Taiwan, R.O.C.
GU076195 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Tracing the origin of Chilades pandava (Lepidoptera, Lycaenidae) found at Kinmen Island using mitochondrial COI and COII genes Unpublished
GU076195 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Direct Submission Submitted (11-OCT-2009) Department of Life Science, National Taiwan Normal University, No. 88, Ting-Chou Rd., Sec. 4, Taipei 116, Taiwan, R.O.C.
GU076194 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Tracing the origin of Chilades pandava (Lepidoptera, Lycaenidae) found at Kinmen Island using mitochondrial COI and COII genes Unpublished
GU076194 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Direct Submission Submitted (11-OCT-2009) Department of Life Science, National Taiwan Normal University, No. 88, Ting-Chou Rd., Sec. 4, Taipei 116, Taiwan, R.O.C.
GU076213 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Tracing the origin of Chilades pandava (Lepidoptera, Lycaenidae) found at Kinmen Island using mitochondrial COI and COII genes Unpublished
GU076213 False Wu,L.-W.
Lees,D.C.
Hsu,Y.-F.
Direct Submission Submitted (11-OCT-2009) Department of Life Science, National Taiwan Normal University, No. 88, Ting-Chou Rd., Sec. 4, Taipei 116, Taiwan, R.O.C.
Show Hide 10 more publications...

Source 6

Hit accession Automated Authors Title Journal
OQ872281 False Nazari,V.
Kunte,K.
Balint,Z.
On the identity of Hesperia parrhasius Fabricius, 1793 and its allied species (Lepidoptera: Lycaenidae) J Asia Pac Entomol 26 (4), 102165 (2023)
OQ872281 False Nazari,V.
Balint,Z.
Direct Submission Submitted (25-APR-2023) Department of Biology, University of Padova, Padova, Italy, P.O. Box 45063, Ottawa, Ontario K2M2Y1, Canada
OQ872255 False Nazari,V.
Kunte,K.
Balint,Z.
On the identity of Hesperia parrhasius Fabricius, 1793 and its allied species (Lepidoptera: Lycaenidae) J Asia Pac Entomol 26 (4), 102165 (2023)
OQ872255 False Nazari,V.
Balint,Z.
Direct Submission Submitted (25-APR-2023) Department of Biology, University of Padova, Padova, Italy, P.O. Box 45063, Ottawa, Ontario K2M2Y1, Canada
OQ872256 False Nazari,V.
Kunte,K.
Balint,Z.
On the identity of Hesperia parrhasius Fabricius, 1793 and its allied species (Lepidoptera: Lycaenidae) J Asia Pac Entomol 26 (4), 102165 (2023)
OQ872256 False Nazari,V.
Balint,Z.
Direct Submission Submitted (25-APR-2023) Department of Biology, University of Padova, Padova, Italy, P.O. Box 45063, Ottawa, Ontario K2M2Y1, Canada
OQ872295 False Nazari,V.
Kunte,K.
Balint,Z.
On the identity of Hesperia parrhasius Fabricius, 1793 and its allied species (Lepidoptera: Lycaenidae) J Asia Pac Entomol 26 (4), 102165 (2023)
OQ872295 False Nazari,V.
Balint,Z.
Direct Submission Submitted (25-APR-2023) Department of Biology, University of Padova, Padova, Italy, P.O. Box 45063, Ottawa, Ontario K2M2Y1, Canada
OQ872294 False Nazari,V.
Kunte,K.
Balint,Z.
On the identity of Hesperia parrhasius Fabricius, 1793 and its allied species (Lepidoptera: Lycaenidae) J Asia Pac Entomol 26 (4), 102165 (2023)
OQ872294 False Nazari,V.
Balint,Z.
Direct Submission Submitted (25-APR-2023) Department of Biology, University of Padova, Padova, Italy, P.O. Box 45063, Ottawa, Ontario K2M2Y1, Canada
Show Hide 8 more publications...

Source 7

Hit accession Automated Authors Title Journal
KT236346 False Xia,X.-Q.
Hao,J.-S.
Direct Submission Submitted (24-MAR-2015) College of Life Sciences, Anhui Normal University, 1 Peking East Road, Wuhu, Anhui 241000, China

Source 8

Hit accession Automated Authors Title Journal
GQ128951 False Vila,R.
Bell,C.D.
Macniven,R.
Goldman-Huertas,B.
Ree,R.H.
Marshall,C.R.
Balint,Z.
Johnson,K.
Benyamini,D.
Pierce,N.E.
Phylogeny and palaeoecology of Polyommatus blue butterflies show Beringia was a climate-regulated gateway to the New World Proc. Biol. Sci. 278 (1719), 2737-2744 (2011)
GQ128951 False Vila,R.
Bell,C.D.
Macniven,R.
Goldman-Huertas,B.M.
Ree,R.H.
Marshall,C.R.
Balint,Z.
Johnson,K.
Benyamini,D.
Pierce,N.E.
Direct Submission Submitted (07-MAY-2009) ICREA and Departament de Genetica i Biologia Molecular, Universitat Autonoma de Barcelona, Edifici C, Bellaterra, Barcelona 08193, Spain

Source 9

Hit accession Automated Authors Title Journal
KJ131016 False Stradomsky,B.
Fomina,E.
Comparative molecular genetic analysis of blue butterflies (Lepidoptera: Lycaenidae) Unpublished
KJ131016 False Stradomsky,B.
Fomina,E.
Direct Submission Submitted (10-JAN-2014) Laboratory of Molecular Biology, Institute of Arid Zones, Chekhov Str., 41, Rostov-on-Don, Rostov 344006, Russia

Source 10

Hit accession Automated Authors Title Journal
KJ402171 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402171 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402247 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402247 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402235 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402235 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
KJ402237 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
DNA barcoding of butterflies from India Unpublished
KJ402237 False Gaikwad,S.S.
Mahale,K.
Ghate,H.V.
Ghaskadbi,S.S.
Shouche,Y.S.
Direct Submission Submitted (03-FEB-2014) Department of Zoology, Modern College, Shivajinagar, Pune, Maharashtra 411005, India
Show Hide 6 more publications...

Source 11

Hit accession Automated Authors Title Journal
KT880664 False Jiji Joseph,V.
Kottickal,L.V.
Molecular characterization of mitochondrial cytochrome oxidase subunit I gene partial coding sequence of the papilionoid butterflies of N. Kerala Unpublished
KT880664 False Jiji Joseph,V.
Kottickal,L.V.
Direct Submission Submitted (06-OCT-2015) Zoology, University of Calicut, Thenhipalam, Dist Malappuram, Kerala 673635 and Govt. Brennen College, Dharmadam, Thalassery, Kerala 670106, India

Source 12

Hit accession Automated Authors Title Journal
ON067494 False Devi,A.
Walia,G.K.
Sidhu,A.K.
Direct Submission Submitted (25-MAR-2022) Zoology and Environmental Sciences, Punjabi University Patiala, Patiala, Patiala, PUNJAB 147002, India

Intraspecies diversity

This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.

Analyst evaluation

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The phylogetic tree was constructed with FastME using the Neighbor-Joining method. Multiple-sequence alignment of the candidate reference sequences was performed using MAFFT. The visualization is rendered with TidyTree.

Taxonomy Check

The following resources can be used to ensure that the given taxonomy is legitimate and current.

Taxa Database
General GBIF
General ITIS
Mealybugs & scale ScaleNet database
Thrips Thripswiki
Spider Mites Spider Mites Database
Psocodea (Barklice, Booklice, and Parasitic Lice) Psocodea Species File Online
Orthoptera Orthoptera Species File Online
Drosophilidae TaxoDros
Diptera Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum
Aphids Aphid Species File
Ants AntWeb
AntCat
Lepidoptera (butterflies and moths) The Global Lepidoptera Names Index
Gracillariidae (primitive moths) Global Taxonomic Database of Gracillariidae
Pyralidae (pyralid moths) Global Information System on Pyraloidea
Tortricidae (tortrix moths) Tortricidae Resources on the Net